| Family | Common Name | Selected Genus and Species | Image |
| Acartophthalmidae | Flies | Acartophthalmus nigrinus |  |
| Acroceridae | Small-headed flies | Acrocera orbiculus |  |
| Agromyzidae | Leaf-miner flies | Napomyza lateralis |  |
| Anthomyiidae | Cabbage flies | Hydrophoria linogrisea |  |
| Anthomyzidae | Flies | Anthomyza pallida |  |
| Apioceridae | Flower-loving flies | Apiocera mexicana |  |
| Apsilocephalidae | Flies | Apsilocephala longistyla |  |
| Apystomyiidae | Flies | Apystomyia elinguis |  |
| Asilidae | Robber flies | Dysmachus trigonus |  |
| Asteiidae | Flies | Asteia amona |  |
| Atelestidae | True flies | Atelestus pulicarius |  |
| Athericidae | Ibis flies | Ibisia marginata |  |
| Aulacigastridae | Sap flies | Aulacigaster leucopeza |  |
| Australimyzidae | Flies | Australimyza australensis |  |
| Austroleptidae | Snipe flies | Austroleptis papaveroi |  |
| Bolbomyiidae | Tiny black flies | Bolbomyia nana |  |
| Bombyliidae | Bee flies | Bombylius major |  |
| Brachystomatidae | Flies | Brachystoma occidentalis |  |
| Braulidae | Bee lice | Braula coeca |  |
| Calliphoridae | Bluebottles | Chrysomya megacephala |  |
| Camillidae | Flies | Camilla nigrifrons |  |
| Canacidae | Beach flies | Tethina lusitanica |  |
| Carnidae | Bird flies | Carnus hemapterus |  |
| Celyphidae | Beetle flies | Acelyphus repletus |  |
| Chamaemyiidae | Flies | Chamaemyia aridella |  |
| Chloropidae | Grass flies | Chlorops pumilionis |  |
| Chyromyidae | Flies | Gymnochiromyia flavella |  |
| Clusiidae | Druid flies | Clusia tigrina |  |
| Coelopidae | Kelp flies | Coelopa pilipes |  |
| Conopidae | Thick-headed flies | Conops quadrifasciatus |  |
| Cryptochetidae | Scale parasite flies | Cryptochetum iceryae |  |
| Ctenostylidae | Flies | Ctenostylum fuscomaculatum |  |
| Curtotonidae | Quasimodo flies | Curtonotum gibbum |  |
| Cypselosomatidae | Flies | Formicosepsis hamata |  |
| Diastatidae | Flies | Diastata fuscula |  |
| Diopsidae | Stalk-eyed flies | Cyrtodiopsis dalmanni |  |
| Dolichopodidae | Long-legged flies | Dolichopus urbanus |  |
| Drosophilidae | Fruit flies | Lordiphosa andalusiaca |  |
| Dryomyzidae | Flies | Dryomyza anilis |  |
| Empididae | Dagger flies | Empis livida |  |
| Ephydridae | Shore flies | Hyadina pulchella |  |
| Evocoidae | Flies | Evocoa chilensis |  |
| Fanniidae | Flies | Fannia canicularis |  |
| Fergusoninidae | Gall-forming flies | Fergusonina microcera |  |
| Glossinidae | Tsetse flies | Glossina morsitans |  |
| Gobryidae | Flies | Gobrya bacchoides |  |
| Helcomyzidae | Flies | Helcomyza ustulata |  |
| Heleomyzidae | Flies | Tephrochlamys rufi |  |
| Helosciomyzidae | Flies | Xenosciomyza turbotti |  |
| Heterocheilidae | Half-bridge flies | Heterocheila buccata |  |
| Heteromyzidae | Flies | Diplogeomyza maculipennis |  |
| Hilarimorphidae | Flies | Hilarimorpha singularis |  |
| Hippoboscidae | Louse flies | Crataerina pallida |  |
| Homalocnemiidae | Flies | Homalocnemis perspicua |  |
| Huttoninidae | Flies | Huttonina furcata |  |
| Hybotidae | Dance flies | Bicellaria spuria |  |
| Inbiomyiidae | Flies | Inbiomyia azevedoi |  |
| Ironomyiidae | Ironic flies | Ironomyia nigromaculata |  |
| Lauxaniidae | Flies | Sapromyza brachysoma |  |
| Lonchaeidae | Lance flies | Lonchaea chorea |  |
| Lonchopteridae | Spear-winged flies | Lonchoptera lutea |  |
| Marginidae | Margin flies | Margo aperta |  |
| Megamerinidae | Flies | Megamerina dolium |  |
| Micropezidae | Stilt-legged flies | Rainieria antennaepes |  |
| Milichiidae | Filth flies | Milichiella arcuata |  |
| Mormotomyiidae | Frightful hairy flies | Mormotomya hirsuta |  |
| Muscidae | House flies | Musca domestica |  |
| Mydidae | Mydas flies | Gauromydas heros |  |
| Mystacinobiidae | New Zealand bat flies | Mystacinobia zelandica |  |
| Mythicomyiidae | Flies | Mythicomyia actites |  |
| Nannodastiidae | Flies | Nannodastia horni |  |
| Natalimyzidae | Flies | Natalimyza milleri |  |
| Nemestrinidae | Tangle-veined flies | Moegistorhynchus longirostris |  |
| Neminidae | Flies | Nemo arbelos |  |
| Neriidae | Cactus flies | Telostylinus lineolatus |  |
| Neurochaetidae | Flies | Neurochaeta inversa |  |
| Nothybidae | Flies | Nothybus biguttatus |  |
| Nycteribiidae | Bat flies | Basilia forcipata |  |
| Odiniidae | Flies | Odinia ornata |  |
| Oestridae | Gadflies | Cephenemyia stimulator |  |
| Opetiidae | Flat-footed flies | Opetia nigra |  |
| Opomyzidae | Flies | Geomyza tripunctata |  |
| Oreogetonidae | Snipe flies | Oreogeton xanthus |  |
| Oreoleptidae | Flies | Oreoleptis torrenticola |  |
| Pallopteridae | Flutter-wing flies | Palloptera umbellatarum |  |
| Pantophthalmidae | Timber flies | Pantophthalmus bellardi |  |
| Paraleucopidae | Flies | Paraleucopis corvina |  |
| Pelecorhynchidae | Flies | Pelecorhynchus darwini |  |
| Periscelididae | Flies | Cyamops alessandrae |  |
| Perissommatidae | Flies | Perissomma mcalpinei |  |
| Phaeomyiidae | Flies | Pelidnoptera fuscipennis |  |
| Phoridae | Coffin flies | Megaselia scalaris |  |
| Piophilidae | Cheese flies | Piophila casei |  |
| Pipunculidae | Big-headed flies | Verrallia aucta |  |
| Platypezidae | Flat-footed flies | Poliporivora picta |  |
| Platystomatidae | Signal flies | Pogonortalis doclea |  |
| Psilidae | Rust flies | Loxocera albiseta |  |
| Pyrgotidae | Picture-winged flies | Pyrgotella chagnoni |  |
| Ragadidae | Flies | Ragas unica |  |
| Rhagionidae | Snipe flies | Rhagio scolopaceus |  |
| Rhiniidae | Flies | Stomorhina lunata |  |
| Rhinophoridae | Woodlouse flies | Melanophora roralis |  |
| Richardiidae | Flies | Sepsisoma flavescens |  |
| Ropalomeridae | Robust flies | Ropalomera femorata |  |
| Sarcophagidae | Flesh flies | Sarcophaga bercaea |  |
| Scathophagidae | Dung flies | Scathophaga stercoraria |  |
| Scenopinidae | Window flies | Scenopinus fenestralis |  |
| Sciomyzidae | Marsh flies | Limnia unguicornis |  |
| Sepsidae | Black scavenger flie | Sepsis fulgens |  |
| Somatiidae | Flies | Somatia schildi |  |
| Sphaeroceridae | Small dung flies | Copromyza equina |  |
| Stratiomyidae | Soldier flies | Hermetia illucens |  |
| Streblidae | Bat flies | Trichobius corynorhini |  |
| Strongylophthalmyiidae | Slender long-legged flies | Strongylophthalmyia ustulata |  |
| Syringogastridae | Ant-mimicking flies | Syringogaster tenuipes. |  |
| Syrphidae | Hover flies | Eristalis tenax |  |
| Tabanidae | Horseflies | Tabanus sulcifrons |  |
| Tachinidae | Parasitic flies | Tachina fera |  |
| Tanypezidae | Stretched-foot flies | Tanypeza longimana |  |
| Tephritidae | Fruit flies | Euaresta aequalis |  |
| Teratomyzidae | Fern flies | Teratomyza undulata |  |
| Therevidae | Stiletto flies | Thereva nobilitata |  |
| Ulidiidae | Picture-winged flies | Tritoxa incurva |  |
| Ulurumyiidae | McAlpine's fly | Ulurumyia macalpinei |  |
| Vermileonidae | Worm-lion flies | Vermileo comstocki |  |
| Xenasteiidae | Flies | Xenasteia sabroskyi |  |
| Xylomyidae | Wood soldier flies | Solva marginata |  |
| Xylophagidae | Awl flies | Coenomyia ferruginea |  |